3D imaging of undissected optically cleared Anopheles stephensi mosquitoes and midguts infected with Plasmodium parasites
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چکیده
منابع مشابه
مقایسه تاثیر آنتی‌بادی‌های ضد میان روده پشه آنوفل استفنسی بر روی چرخه اسپروگونی انگل پلاسمودیوم برگئی در دو سویه از آنوفل استفنسی
Background & Aims: Malaria parasites attach to molecules on midgut surface of Anopheles mosquitoes to continue their life cycle. Here we try to evaluate the effect of anti-Anopheles midgut antibodies on coating these molecules and consequently blocking the transmission cycle of Plasmodium berghei inside their vectors. Materials & Methods: Balb/c mice were immunized with homogenized and degly...
متن کاملPlasmodium falciparum-infected Anopheles stephensi inconsistently transmit malaria to humans.
Malaria was transmitted to only 5 of 10 volunteers bitten by 1-2 Anopheles stephensi carrying sporozoites of the 3D7 clone of the NF54 strain of Plasmodium falciparum in their salivary glands. Parasites were detectable by culture in blood taken 7-10 days following exposure and by thick blood film 14-16.5 days after exposure. Infectivity did not correlate with the numbers of sporozoites in the s...
متن کاملLaser induced mortality of Anopheles stephensi mosquitoes.
Small, flying insects continue to pose great risks to both human health and agricultural production throughout the world, so there remains a compelling need to develop new vector and pest control approaches. Here, we examined the use of short (<25 ms) laser pulses to kill or disable anesthetized female Anopheles stephensi mosquitoes, which were chosen as a representative species. The mortality ...
متن کاملThe Maternally Inheritable Wolbachia wAlbB Induces Refractoriness to Plasmodium berghei in Anopheles stephensi
The endosymbiont Wolbachia wAlbB induces refractoriness to Plasmodium falciparum in Anopheles stephensi, the primary mosquito vector of human malaria in the Middle East and South Asia. However, it remains unknown whether such refractoriness can be extended to other malaria species. In particular, it was reported that under very specific conditions, wAlbB can enhance Plasmodium infection in some...
متن کاملProgression of Plasmodium berghei through Anopheles stephensi Is Density-Dependent
It is well documented that the density of Plasmodium in its vertebrate host modulates the physiological response induced; this in turn regulates parasite survival and transmission. It is less clear that parasite density in the mosquito regulates survival and transmission of this important pathogen. Numerous studies have described conversion rates of Plasmodium from one life stage to the next wi...
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ژورنال
عنوان ژورنال: PLOS ONE
سال: 2020
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0238134